Studies of cells cultured in biomimetic environment are leading issues in current cell biology. Bio-MEMS (including microfluidics) is the appropriate technique to construct specific condition into one small system extracting a target in vivo environment. It can provide controlling spatiotemporal condition on the micrometer scale 2D and 3D cell culture system. In addition, it can test variable condition in parallel with high throughput in a small device simultaneously. Reducing time, space and material consumption is also big advantage of this technique. Overcoming the limitation of conventional in vitro experiment, it has improved the reliability of the test. According to the flexible and extensive applicability and high efficiency, the authentic in vitro system has become one of major trends in the field of cell and tissue engineering.

Tissue engineering applies the principles and methods of life sciences, bioengineering, and material science toward the assembly of biologic substitutes that will restore, maintain and improve tissue functions following damage either by disease or traumatic process. Currently, we are developing a smart hydrogel for angiogenesis and revascularization of damage tissue through the 3-dimensional cell culture and in vivo study base on tissue engineering.